Genetic dissection of auxin signalling in Arabidopsis thaliana

by Sally P. Ward

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Written in English
Published: Pages: 287 Downloads: 232
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Thesis (Ph.D.) - University of Warwick, 1997.

StatementSally P. Ward.
The Physical Object
Number of Pages287
ID Numbers
Open LibraryOL17488795M

  Mutant lines of Arabidopsis thaliana resistant to the artificial auxin 2,4-dichloro phenoxyacetic acid (2,4-D) were isolated by screening for growth of seedlings in the presence of toxic levels of 2,4-D. Genetic analysis of these resistant lines indicated that 2,4-D resistance is due to a recessive mutation at a locus we have designated AxrCited by: The genetic identification of Arabidopsis auxin-resistant mutants has provided crucial insight into the molecular mechanisms underlying auxin signaling. Molecular and biochemical studies of the gene products that are affected by these mutations have positioned the SCF TIR1 ubiquitin-ligase complex as a central regulator of auxin signaling. We investigated a genetic pathway in root hair development in Arabidopsis thaliana, involving the receptor‐like kinase FERONIA (FER), two guanine nucleotide exchange factors for ROP s (RopGEF 4 and RopGEF 10), and the small GTP ase Rho of plants (ROP s).; Loss‐ and gain‐of‐function analyses demonstrated distinct roles of RopGEF 4 and RopGEF 10 such that RopGEF 4 is only important for Cited by: The role of auxin during pattern formation in Arabidopsis thaliana 5 Abbreviations 2,4-D 2,4-dichlorophenoxyacetic acid AFB AUXIN SIGNALING F BOX PROTEIN ARE AUXIN RESPONSIVE ELEMENT ARFs AUXIN RESPONSE FACTORs ATP Adenosine triphosphate Aux/IAA INDOLEACETIC ACID INDUCIBLE BDL BODENLOS Ca2+ Calcium.

  The TIR1/AFB proteins are auxin co-receptors that mediate diverse responses to the plant hormone auxin. The genome of Arabidopsis encodes six TIR1/AFB proteins representing three of the four clades that were established prior to angiosperm radiation. To determine the role of these proteins in plant growth and development we have performed an extensive genetic analysis involving the Cited by: 2. Auxin signaling Tomasz Paciorek and Arabidopsis abp1 loss-of-function mutant is lethal (Chen et al., ), genetic evidence for the involvement of ABP1 in auxin-dependent development is hard to obtain. Thus, the significance of its binding to auxin remains unclear. Forward genetic screens identified a group of Arabidopsis mutants able to. Molecular Plant • Volume 1 • Number 3 • Pages – • May RESEARCH ARTICLE Repression of the Auxin Response Pathway Increases Arabidopsis Susceptibility to Necrotrophic Fungi Francisco Llorentea, Paul Muskettb, Andrea Sa´nchez-Valleta, Gemma Lo´peza, Brisa Ramosa, Clara Sa´nchez-Rodrı´gueza, Lucia Jorda´a, Jane Parkerb and Antonio Molinaa,1. auxin response patterns in developing Arabidopsis leaves as well as Arabidopsis mutants and transgenic plants to trace pathways of auxin signal transduction controlling the expression of early procambial genes. We show that in young Arabidopsis leaf primordia, molecular auxin response patterns presage sites of procambial differentiation.

Auxin is an important plant hormone responsible for plant growth and development. This hormone regulates gene expression which is dependent on three families of proteins as described above [4]. While some studies have described the role of this gene regulatory network on auxin-mediated responses, detailed analysis of this gene family was lacking. Epigenetic regulation involves a myriad of mechanisms that regulate the expression of loci without altering the DNA sequence. These different mechanisms primarily result in modifications of the chromatin topology or DNA chemical structure that can be heritable or transient as a dynamic response to environmental cues. The phytohormone auxin plays an important role in almost every aspect of Author: Eduardo Mateo-Bonmatí, Rubén Casanova-Sáez, Karin Ljung.

Genetic dissection of auxin signalling in Arabidopsis thaliana by Sally P. Ward Download PDF EPUB FB2

Control of seed size involves complex interactions among the zygotic embryo and endosperm, the maternally derived seed coat, and the parent plant. Here we describe a mutant in Arabidopsis, megaintegumenta (mnt), in which seed size and weight are dramatically increased. One factor in this is extra cell division in the integuments surrounding mnt mutant ovules, leading to the Cited by:   The first major advance in the genetic dissection of auxin biosynthesis in plants was the isolation of the auxin overproduction mutants superroot1 (sur1: At2g) and superroot2 (sur2: At4g) in Arabidopsis (Boerjan et al., ; Delarue et al., ).

When grown in light, both sur1 and sur2 have much longer hypocotyls than do wild type. The AUXIN RESPONSE FACTOR 2 gene of Arabidopsis links auxin signalling, cell division, and the size of seeds and other organs Marie C. Schruff 1, Melissa Spielman, Sushma Tiwari, Sally Adams 2, Nick Fenby 1 and Rod J.

Scott 1,* Control of seed size involves complex interactions among the zygotic embryo and endosperm, the maternally derived. To understand how auxin regulates root growth, we quantified cell division and elemental elongation, and examined actin organization in the primary root of Arabidopsis treatments for 48 h that inhibited root elongation rate by 50%, we find that auxins and auxin‐transport inhibitors can be divided into two classes based on their effects on cell division, elongation and actin Cited by: Auxin, actin and growth of the Arabidopsis thaliana primary root Abidur Rahman1,2, Alex Bannigan1, Waheeda Sulaman1, Priit Pechter3, Elison B.

Blancaflor3 and Tobias I. Baskin1,* 1Biology Department, University of Massachusetts, Amherst, MAUSA, 2Cryobiosystem Research Center, Faculty of Agriculture, Iwate University, MoriokaJapan, andCited by:   This protocol allows the measurement of auxin transport in roots, hypocotyls and inflorescences of Arabidopsis thaliana plants by examining transport of Cited by:   In Arabidopsis thaliana, a newly identified auxin signalling pathway that involves TMK1 protein cleavage and IAA32 and IAA34 transcriptional Cited by: The AUXIN RESPONSE FACTOR 2 gene of Arabidopsis links auxin signalling, cell division, and the size of seeds and other organs Article (PDF Available) in Development (2) February   lndoleacetic acid (IAA), the most important natural auxin in plants, is mainly synthesized from the amino acid tryptophan (Trp).

Recent genetic and biochemical studies in Arabidopsis have unambiguously established the first complete Trp-dependent auxin biosynthesis pathway. The first chemical step of auxin biosynthesis is the removal of the amino group from Trp by the TRYPTOPHAN.

A number of observations have implicated auxin in the formation of vascular tissues in plant organs. These include vascular strand formation in response to local auxin application, the effects of impaired auxin transport on vascular patterns and suggestive phenotypes of Arabidopsis auxin response mutants.

In this study, we have used molecular markers to visualize auxin response patterns in. The phytohormone auxin plays critical roles in the regulation of plant growth and development. Indoleacetic acid (IAA) has been recognized as the major auxin for more than 70 y.

Although several pathways have been proposed, how auxin is synthesized in plants is still by: Functional Genomic Analysis of the AUXIN/INDOLEACETIC ACID Gene Family Members in Arabidopsis thaliana W Paul J.

Overvoorde,a,1 2 Yoko Okushima,a,1 3 Jose´ M. Alonso,b 4 April Chan,a Charlie Chang,a Joseph R. Ecker,b Beth Hughes,a Amy Liu,a Courtney Onodera,a Hong Quach,a Alison Smith,a Guixia Yu,a and Athanasios Theologisa,5 a Plant Gene Expression Center, Cited by:   A deep mechanistic understanding of auxin signaling proteins therefore may allow rational engineering of novel plant architectures.

Toward that end, we analyzed natural variation in the auxin receptor F-box family of wild accessions of the reference plant Arabidopsis thaliana and used this information to populate a structure/function by: 9. All Arabidopsis (Arabidopsis thaliana) plants used in this study were in the Columbia-0 background.

The T-DNA insertion lines SALK_ (als), SALK_ (stop1), SALK_C (almt1), SALK_ (lpr1), and CS (star1) were obtained from the Arabidopsis Biological Resource by: 9. Here, we show that exposure of Arabidopsis thaliana roots to Al induces a localized enhancement of auxin signaling in the root-apex TZ that is dependent on TAA1, which encodes a Trp.

Forward genetic screens identified a group of Arabidopsis mutants able to elongate roots despite the presence of inhibitory auxin levels [auxin-resistant (axr) mutants]. The molecular characterization of the axr1 mutant revealed that AXR1 function is related to the ubiquitylation pathway, which can mark proteins for proteasome-mediated.

Bimodular auxin response controls organogenesis in Arabidopsis Arabidopsis thaliana, lateral root development is a classic example of a developmental process that is controlled by auxin at multiple stages.

Therefore, we used lateral root formation as a model sys- that although genetic stimulation of the basic cell cycle machi-Cited by:   The phytohormone auxin plays critical roles in the regulation of plant growth and development.

Indoleacetic acid (IAA) has been recognized as the major auxin for more than 70 y. Although several pathways have been proposed, how auxin is synthesized in plants is still unclear. Previous genetic and enzymatic studies demonstrated that both TRYPTOPHAN Cited by: new publication search check the boxes below and download results Your query for publications where locus name is AT3G resulted in 50 matches.

Ethylene represents an important regulatory signal for root development. Genetic studies in Arabidopsis thaliana have demonstrated that ethylene inhibition of root growth involves another hormone signal, auxin.

This study investigated why auxin was required by ethylene to regulate root growth. We initially observed that ethylene positively controls auxin biosynthesis in the root apex. Untargeted LC-MS-based metabolomic approaches and quantitative genetic analysis enabled broad-spectrum molecular dissection of the Arabidopsis leaf metabolite composition of Cvi×Ler RILs (Keurentjes et al., ).

Mapping of > mass peaks resulted in the identification of mQTLs for ~75% of all mass signals, which is comparable with the Cited by: Arabidopsis thaliana, the thale cress, mouse-ear cress or arabidopsis, is a small flowering plant native to Eurasia and Africa.

thaliana is considered a weed; it is found by roadsides and in disturbed land. A winter annual with a relatively short life cycle, A. thaliana is a popular model organism in plant biology and genetics. For a complex multicellular eukaryote, A.

thaliana has a Family: Brassicaceae. Calossin-like protein required for polar auxin transport. Involved in regulating sugar response and C/N balance. Genetic dissection of Fe-dependent signaling in root developmental responses to phosphate deficiency Mitochondrial Function Modulates Touch Signalling in Arabidopsis thaliana.

Auxin research has reached a watershed. The first plant genes encoding products that play a role in auxin action (as defined by genetic analysis) have been cloned. Although the sequences of the genes have not revealed functions in the cellular response to auxin, they have opened a tantalizing image of potential elements of auxin signal by:   Here the current status of efforts to understand auxin homeostatic mechanisms during development of the gynoecium in Arabidopsis thaliana are reviewed.

Gynoecial structure The mature gynoecium is a complex structure with different organs, tissues, and cell types that are organized to function in concert to support female reproductive by: As plants are sessile organisms, their developmental plasticity is crucial for adapting to environmental signals.

Plant roots play a key role foraging resources in the soil to facilitate uptake of water and nutrients. The plant hormone auxin is a critical regulator of root growth and development, controlling root hair, branching and root by: 1. Genetic dissection of auxin signalling in Arabidopsis thaliana.

(Thesis) Ward SP. Publisher: University of Warwick [] Metadata Source: The British Library Type: Thesis. Abstract.

Highlight Terms No biological terms identified No abstract supplied. Menu Formats. Abstract; Thesis at EThOS. The axr Mutation of Arabidopsis thaliana Is a Gain-of-Function Mutation That Disrupts an Early Step in Auxin Response Candace Timpte, Allison K.

Wilson' and Mark Estelle Department of Biology, Indiana University, Bloomington, Indiana Manuscript received J Accepted for publication Aug ABSTRACTCited by: Analysis of auxin and abscisic acid signal transduction in Arabidopsis thaliana Yulin Tang Vollständiger Abdruck der von der Fakultät Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt der Technischen Universität München zur Erlangung des akademischen Grades eines Doktors der Naturwissenschaften (Dr.

rer. nat.). Genetic dissection of IAA biosynthesis has proven difficult, and no IAA-deficient mutants have been identified. Many of the difficulties in dissecting IAA biosynthesis by using genetics have been attributed to redundancy between and within these various proposed IAA biosynthesis pathways (Fig.

1).Although labeling studies indicate that both tryptophan (Trp)-dependent and Trp-independent IAA. Quantitative genetic analysis of auxin-driven growth in Arabidopsis thaliana Jennifer Wright Master of Science (by Research) University of York Biology September 2 Growth of Arabidopsis thaliana 11 Auxin 12 Warmth 14 HSP   The basipetal auxin distribution profile is modified in the trh1 auxin transport mutant of Arabidopsis thaliana.

Concentration‐ and time‐dependent elevation of auxin accumulation in wild‐type (a) and trh1 (b) roots following the expression pattern of the synthetic auxin response transgene DR by: ALI / Turk J Bot 2 lines aux (insensitive to auxin and ethylene), axr (insensitive to auxin), and eir (insensitive to ethylene).

Bacterial strains of the genera, Pseudomonas, Bacillus Micrococcus, Escherichia, and Staphylococcus that showed in vitro auxin production were used to inoculate Arabidopsis thaliana.